How can I elute my HaloTag fusion protein?.Can I pulldown a HaloTag fusion protein, that is already bound to a substrate?.How can I immunoprecipitate my Halo-tagged protein?.
How can I detect my Halo-tagged protein in Western blot?.What are the differences between HaloTag and GFP?.What are the differences between HaloTag and Snap-Tag?.Is there more than one HaloTag version available?.For what applications can HaloTag be used?.How is the complex between the HaloTag and a ligand formed?.Sticky ends from different BlpI sites may not be compatible. PshAI quickly loses activity at 37☌, but can be used at 25☌ for long incubations.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence.īssHII is typically used at 50☌, but is 75% active at 37☌. Sticky ends from different PasI sites may not be compatible. This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. Prolonged incubation with BseRI may lead to degradation of the DNA.
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